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Patel, Rakesh K.
- Estimation of Total Phenolics and Flavonoids and Antioxidant Potential of Drakshasava Prepared by Traditional and Modern Methods
Abstract Views :332 |
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Authors
Affiliations
1 Head of Department of Pharmacognosy and Phytochemistry, IIMT College of Medical Sciences, Meerut (U.P.), IN
2 Head of Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat, IN
1 Head of Department of Pharmacognosy and Phytochemistry, IIMT College of Medical Sciences, Meerut (U.P.), IN
2 Head of Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat, IN
Source
Asian Journal of Research in Chemistry, Vol 6, No 3 (2013), Pagination: 204-208Abstract
The objective of the present study was to estimate the total phenolic content as well as flavonoids in Drakshasava-T and Drakshasava-M prepared by traditional and modern methods respectively and in its marketed preparation and also to evaluate the antioxidant activity of these test preparations on two different in vitro antioxidant activity models. Total phenolic content was determined colorimetrically using Folin-Ciocalteu reagent and was found 0.1045 and 0.1041 %w/w gallic acid equivalent in Drakshasava-T and Drakshasava-M respectively. Total flavonoid content was determined by aluminium chloride method and was found 0.01264 and 0.01214 %w/w quercetin equivalent in Drakshasava-T and Drakshasava-M respectively. Super-oxide anion scavenging activity and lipid per-oxidation assay were carried out to evaluate the antioxidant potential of Drakshasava-T and Drakshasava-M. The antioxidant activity of Drakshasava-T and Drakshasava-M was found increased in concentration dependent manner in both the in vitro antioxidant activity models as super-oxide radical scavenging activity and lipid per-oxidation assay. Drakshasava-T and Drakshasava-M showed significant scavenging of super-oxide radical and showed IC50 120.63 and 128.36 μg/ml respectively. Drakshasava-T and Drakshasava-M also inhibited the ferrous sulphate induced lipid per-oxidation in dose dependent manner and showed inhibitory concentration (IC50) 212.50 and 220.15 μg/ml respectively. Marketed Drakshasava also showed a rich concentration of total phenolics and flavonoids and showed dose dependent antioxidant activity in both the models. Thus, the results obtained in this study indicate that Drakshasava-T and Drakshasva-M can be a promising source of natural antioxidant.Keywords
Total Phenolics, Flavonoids, Antioxidant Potential, Drakshasava
References
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- Akoh CC, Bonilla EP, Sellappan S, Krewer G. Phenolic content and antioxidant capacity of Muscadine grapes. Journal of Agricultural and Food Chemistry 2003; 51:5497-5503.
- Frankel EN, Kanner J, German JB, Parks E, Kinsella JE. Inhibition of oxidation of human low-density lipoprotein by phenolic substances in red wine. The Lancet 1993; 341(20):454- 457.
- Mayer AS, Yi OS, Person DA, Waterhouse DL, Frankel EN. Inhibition of human low density lipoprotein oxidation in relation to composition of phenolic antioxidants in grapes (Vitis vinifera). Journal of Agricultural and Food Chemistry 1997; 45:1638-1643.
- Teissedre PL, Frankel EN, Waterhouse AL, Peleg H, German GB. Inhibition of in vitro human LDL oxidation by phenolic antioxidants from grapes and wines. Journal of the Science of Food and Agriculture 1996; 70:55-61.
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- Davalos A, Bortolome B, Gomez-cordoves C. Antioxidant properties of commercial grape juices and vinegars. Food Chemistry 2005; 93(2):325-330.
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- Development and Validation of HPTLC Method for Quantification of Gallic acid and Catechin from Draksharishta
Abstract Views :311 |
PDF Views:1
Authors
Affiliations
1 Department of Pharmacognosy, Shri Sarvajanik Pharmacy College, Mehsana-384001, Gujarat, IN
2 Department of Pharmaceutical Chemistry, Shri Sarvajanik Pharmacy College, Mehsana-384001, Gujarat, IN
3 Head of Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat, IN
1 Department of Pharmacognosy, Shri Sarvajanik Pharmacy College, Mehsana-384001, Gujarat, IN
2 Department of Pharmaceutical Chemistry, Shri Sarvajanik Pharmacy College, Mehsana-384001, Gujarat, IN
3 Head of Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat, IN
Source
Asian Journal of Research in Chemistry, Vol 6, No 3 (2013), Pagination: 248-253Abstract
A simple, precise and accurate HPTLC method has been established for the determination of gallic acid and catechin in test formulations of Draksharishta as Draksharishta-T and Draksharishta-M prepared by traditional and modern method respectively and also in its marketed formulation. Draksharishta is a polyherbal hydro alcoholic preparation and is used to improve digestion, as blood purifier, in the treatment of anaemia and advised as a choice of remedy in respiratory problems. The developed HPTLC method was validated in terms of precision, accuracy, LOD, LOQ and specificity. The amount of gallic acid in Draksharishta-T, M and in its marketed formulation was found to be 0.0227, 0.0225 and 0.0226 % w/w respectively while catechin was found to be 0.0176, 0.0175 and 0.0175 %w/w respectively. Quantification of gallic acid and catechin in Draksharishta has been reported first time by validated HPTLC. Furthermore, no TLC densitometric methods have been reported for the quantification of gallic acid and catechin from Draksharishta.Keywords
Draksharishta, HPTLC, Validation, Gallic Acid, CatechinReferences
- The Ayurvedic Formulary of India Part-I, Controller of Publications, Delhi; 2000:15-16.
- Baydar NG, Ozkan G, Sagdic O. Total phenolic contents and antibacterial activities of grape (Vitis vinifera L.) extracts. Food Control 2004; 15: 335-339.
- Frankel EN, Kanner J, German JB, parks E, Kinsella JE. Inhibition of oxidation of human low density lipoprotein by phenolic substances in red wine. Lancet 1993; 341 (20): 454-457.
- Mayer AS, Yi OS, Person DA, waterhouse DL, Frankel EN. Inhibition of human low density lipoprotein oxidation in relation to composition of phenolic antioxidants in grapes (Vitis vonofera). Journal of Agriculture and Food Chemistry 1997; 45: 1638-1643.
- Teissure PL, Frankel EN, Waterhouse AL, Peleg H, German GB. Inhibition of in vitro human LDL oxidation by phenolic antioxidants from grapes and wines. Journal of Science Food and Agriculture 1996; 70:55-61.
- Waterhouse AL. Wine antioxidants may reduce heart disease and cancer. Presentation of American Chemical Society, Washington; 1994.
- Mishra S, Bhaisazya Kalpana Vigyan. Varanasi (India): Chaukambha Surbharati Prakashan; 2005:253-254.
- Alam M, Radhamani S, Ali U, Puroshottam KK. Microbiological screening of dhataki flowers. Journal of research in Ayurveda and Siddha 1984; 2(4): 371-375.
- ICH guidelines Q2 (R1). Validation of analytical procedures. Methodology, Geneva, 2006.
- Singh B, Mungara P, Nivsarkar M, Anandjiwala S. HPTLC densitometry quantification of glycyrrhizin, glycyrrhizinic acid, apigenin, kaempferol and quercetin from Glycyrrhiza glabra. Chromatographia 2009; 70: 1665-1672.
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- Evaluation of Antihyperlipidemic Potential of Drakshasava Prepared by Traditional and Modern Methods in Hyperlipidemic Rats
Abstract Views :653 |
PDF Views:2
Authors
Affiliations
1 Head of Department of Pharmacognosy and Phytochemistry, IIMT College of Medical Sciences, Meerut, (U.P.), IN
2 Head of Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat, IN
1 Head of Department of Pharmacognosy and Phytochemistry, IIMT College of Medical Sciences, Meerut, (U.P.), IN
2 Head of Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat, IN
Source
Research Journal of Pharmacology and Pharmacodynamics, Vol 5, No 2 (2013), Pagination: 92-97Abstract
The objective of the present study was to evaluate the lipid peroxidation activity and related antihyperlipidemic activity of Drakshasava-T and Drakshasava-M prepared by traditional and modern methods and its marketed formulation in high fat diet induced hyperlipidemic rats. The antioxidant activity of Drakshasava-T and Drakshasava-M was increased in concentration dependent manner. Drakshasava-T and Drakshasava-M inhibited the ferrous sulphate induced lipid peroxidation in a dose dependent manner and showed inhibitory concentration (IC50) value 212.50 and 220.15 μg/ml respectively. Oral administration of Drakshasava-T and Drakshasava- M for nine weeks at the dose of 2 ml/kg significantly reduced serum cholesterol, serum LDL and serum triglycerides while showed significant rise in serum HDL as compared to high fat diet fed control group. Marketed Drakshasava also showed significant decrease in serum cholesterol, serum LDL, serum triglycerides and showed significant rise in serum HDL. Atorvastatin (1.2 mg/kg, p.o.) was used as standard antihyperlipidemic drug. Both types of Drakshasava as Drakshasava-T and Drakshasava-M showed significant reduction in atherogenic index as compared to high fat diet fed control group which strongly supports antiatherosclerotic property of Drakshasava.Keywords
Drakshasava, Lipid per Oxidation, Atherogenic Index, Antihyperlipidemic Activity, AtorvastatinReferences
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- The Ayurvedic Formulary of India, Part-II, 2000, 1st edition, The Controller of Publications, Delhi, p.35.
- Baydar NG, Ozkan G, Sagdic O. Total phenolic contents and antibacterial activities of grape (Vitis vinifera L.) extracts. Food Control 2004; 15:335-339.
- Akoh CC, Bonilla EP, Sellappan S, Krewer G. Phenolic content and antioxidant capacity of Muscadine grapes. Journal of Agricultural and Food Chemistry 2003; 51:5497-5503.
- Frankel EN, Kanner J, German JB, Parks E, Kinsella JE. Inhibition of oxidation of human low-density lipoprotein by phenolic substances in red wine. The Lancet 1993; 341(20):454- 457.
- Mayer AS, Yi OS, Person DA, Waterhouse DL, Frankel EN. Inhibition of human low density lipoprotein oxidation in relation to composition of phenolic antioxidants in grapes (Vitis vinifera). Journal of Agricultural and Food Chemistry 1997; 45:1638-1643.
- Teissedre PL, Frankel EN, Waterhouse AL, Peleg H, German GB. Inhibition of in vitro human LDL oxidation by phenolic antioxidants from grapes and wines. Journal of the Science of Food and Agriculture 1996; 70:55-61.
- Waterhouse AL. Wine antioxidants may reduce heart disease and cancer. Presentation of American Chemical Society, Washington; 1994.
- Renaud S, Lorgeril MD. Wine, alcohol, platelets and the French paradox for coronary heart disease. The Lancet 1992; 339:1523- 1526.
- Davalos A, Bortolome B, Gomez-cordoves C. Antioxidant properties of commercial grape juices and vinegars. Food Chemistry 2005; 93(2):325-330.
- Orhan DD, Orhan N, Ergun E, Ergun F. Hepatoprotective effect of Vitis vinifera L. leaves on carbon tetrachloride-induced acute liver damage in rats. Jornal of Ethnopharmacology 2007; 112:145-151.
- Corder R, Mullen W, Khan NQ, Marks SC, Wood EG, Carrier MJ, Crozier A. Red wine procyanidins and vascular health. Nature 2006;444:566.
- Mishra S. Bhaisazya Kalpana Vigyan. Varanasi, India: Chaukambha Surbharati Prakashan; 2005.p. 253-254.
- Alam M, Radhamani S, Ali U, Purushottam KK. Microbiological Screening of Dhataki Flowers. Journal of Research in Ayurveda and Siddha 1984; 2(4):371-375.
- Braugghler JM, Duncan CA and Chase IR. The involvement of iron in lipid peroxidation. J Biol Chem. 1986; 261(22): 10282- 89.
- Lowery OH, Rosenbrough NJ, Farr AL and Randall RJ. Protein estimation with Folin phenol reagent. Biol Chem. 1951;193:265-275.
- Khanna AK, Chander R and Kapoor NK. Terminala arjuna: an Ayurvedic cardiotonic, Regulates lipid metabolism in Hyperlipidemic rats. Phytother Res. 1996;10: 663-665.
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- Evaluation of Diuretic Potential of Drakshasava Prepared by Traditional and Modern Methods in Experimental Albino Rats
Abstract Views :225 |
PDF Views:0
Authors
Affiliations
1 Department of Pharmacognosy, Shri Sarvajanik Pharmacy College, Mehsana-384001, Gujarat, IN
2 Head of Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat, IN
1 Department of Pharmacognosy, Shri Sarvajanik Pharmacy College, Mehsana-384001, Gujarat, IN
2 Head of Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat, IN
Source
Research Journal of Pharmacology and Pharmacodynamics, Vol 4, No 5 (2012), Pagination: 281-284Abstract
The objective of the present study was to evaluate the diuretic potential of Drakshasava-T and Drakshasava-M prepared by traditional and modern methods respectively and its marketed formulation in experimental rats using furosemide (10 mg/kg p.o.) as a standard diuretic drug. Oral administration of Drakshasava-T, Drakshasava-M and its marketed formulation at the dose of 2.0 ml/kg over a period of 5 h showed a significant increase in urine volume as compared to control group. Both types of Drakshasava as Drakshasava-T and Drakshasava-M prepared by traditional and modern methods respectively and its marketed formulation showed significant increase in sodium, potassium and chloride level in urine sample as compared to control group. The maximum diuretic effect was produced by furosemide. Thus, both types of Drakshasava as Drakshasava-T and Drakshasava-M and its marketed formulation showed significant diuretic, natriuretic and kaliuretic effects.Keywords
Diuretic Potential, Furosemide, Drakshasava, Natriuretic Effect, Kaliuretic Effect.References
- The Ayurvedic Formulary of India, Part-II, 2000, 1st edition, The Controller of Publications, Delhi, p.35.
- Baydar NG, Ozkan G, Sagdic O. Total phenolic contents and antibacterial activities of grape (Vitis vinifera L.) extracts. Food Control 2004; 15:335-339.
- Akoh CC, Bonilla EP, Sellappan S, Krewer G. Phenolic content and antioxidant capacity of Muscadine grapes. Journal of Agricultural and Food Chemistry 2003; 51:5497-5503.
- Frankel EN, Kanner J, German JB, Parks E, Kinsella JE. Inhibition of oxidation of human low-density lipoprotein by phenolic substances in red wine. The Lancet 1993; 341(20):454- 457.
- Mayer AS, Yi OS, Person DA, Waterhouse DL, Frankel EN. Inhibition of human low density lipoprotein oxidation in relation to composition of phenolic antioxidants in grapes (Vitis vinifera). Journal of Agricultural and Food Chemistry 1997; 45:1638-1643.
- Teissedre PL, Frankel EN, Waterhouse AL, Peleg H, German GB. Inhibition of in vitro human LDL oxidation by phenolic antioxidants from grapes and wines. Journal of the Science of Food and Agriculture 1996; 70:55-61.
- Waterhouse AL. Wine antioxidants may reduce heart disease and cancer. Presentation of American Chemical Society, Washington; 1994.
- Renaud S, Lorgeril MD. Wine, alcohol, platelets and the French paradox for coronary heart disease. The Lancet 1992; 339:1523- 1526.
- Davalos A, Bortolome B, Gomez-cordoves C. Antioxidant properties of commercial grape juices and vinegars. Food Chemistry 2005; 93(2):325-330.
- Orhan DD, Orhan N, Ergun E, Ergun F. Hepatoprotective effect of Vitis vinifera L. leaves on carbon tetrachloride-induced acute liver damage in rats. Jornal of Ethnopharmacology 2007; 112:145-151.
- Corder R, Mullen W, Khan NQ, Marks SC, Wood EG, Carrier MJ, Crozier A. Red wine procyanidins and vascular health. Nature 2006;444:566.
- Mishra S. Bhaisazya Kalpana Vigyan. Varanasi, India: Chaukambha Surbharati Prakashan; 2005.p. 253-254.
- Alam M, Radhamani S, Ali U, Purushottam KK. Microbiological Screening of Dhataki Flowers. Journal of Research in Ayurveda and Siddha 1984; 2(4):371-375.
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- Cardioprotective Activity of Ashwagandharishta on Isoproterenol Induced Myocardial Infarction
Abstract Views :243 |
PDF Views:0
Authors
Affiliations
1 Department of Pharmacognosy, Shri Sarvajanik Pharmacy College, Mehsana-384001, Gujarat, IN
2 Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat, IN
1 Department of Pharmacognosy, Shri Sarvajanik Pharmacy College, Mehsana-384001, Gujarat, IN
2 Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat, IN
Source
Research Journal of Pharmacology and Pharmacodynamics, Vol 4, No 5 (2012), Pagination: 294-298Abstract
The present study was designed to evaluate the cardio protective activity of Ashwagandharishta-T, Ashwagandahrishta-M prepared by traditional and modern methods respectively and its marketed preparation on isoproterenol (ISO) induced myocardial infarction (MI) in albino rats. Wistar albino rats of either sex were randomly divided into 06 groups comprising 06 animals in each group as normal control, ISO control, pretreatment with Inderal*10 (10 mg/kg) per os, pretreatment with Ashwagandharishta-T, M and its marketed preparation at the dose of 2 ml/kg per os per day for 30 days. MI was induced in all the groups except normal control, by administering ISO (85 mg/kg) intraperitoneally, on 29th and 30th day. On 31st day, level of serum marker enzymes was determined and serum lipid profile was also measured. Then, animals were subsequently sacrificed, hearts were removed, weighed and immediately processed for biochemical studies. Pretreatment with Inderal*10 and all the test preparations of Ashwagandharishta significantly prevented the ISO-induced adverse changes in the level of serum marker enzymes as creatine kinase (CK-MB), lactate dehydrogenase (LDH), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and also improved serum lipid profile. All the test formulations pretreated groups showed significant increase in glutathione (GSH) content and significantly reduced malonyldialdehyde (MDA). Thus, experimental finding suggests that the cardio protective activity of Ashwagandharishta-T, M and its marketed preparation may be due to an augmentation of endogenous antioxidants as GSH and inhibition of lipid peroxidation of cardiac membrane.Keywords
Myocardial Infarction, Isoproterenol, Ashwagandharishta.References
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- Pederson TR. Low density lipoprotein cholesterol lowering is and will be the key to the future of lipid management. American Journal of Cardiology 2001; 87(5A):8B-12B.
- Bolden WE, Pearson TA. Raising low levels of High density lipoprotein cholesterol is an important target of therapy. American Journal of Cardiology 2000; 85(5):645-50.
- Estimation of Total Phenolics and Flavonoids and Antioxidant Potential of Ashwagandharishta Prepared by Traditional and Modern Methods
Abstract Views :195 |
PDF Views:0
Authors
Affiliations
1 Department of Pharmacognosy and Phytochemistry, IIMT College of Medical Sciences, Meerut (U.P.), IN
2 Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat,, ID
1 Department of Pharmacognosy and Phytochemistry, IIMT College of Medical Sciences, Meerut (U.P.), IN
2 Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat,, ID
Source
Asian Journal of Pharmaceutical Analysis, Vol 3, No 4 (2013), Pagination: 147-152Abstract
The objective of the present study was to estimate the total phenolic content as well as flavonoids in Ashwagandharishta-T and Ashwagandharishta-M prepared by traditional and modern methods respectively and in its marketed preparation and also to evaluate the antioxidant activity of these test preparations on two different in vitro antioxidant activity models. Total phenolic content was determined colorimetrically using Folin-Ciocalteu reagent and was found 0.1068 and 0.1065 %w/w gallic acid equivalent in Ashwagandharishta-T and Ashwagandharishta-M respectively. Total flavonoid content was determined by aluminium chloride method and was found 0.01366 and 0.01315 %w/w quercetin equivalent in Ashwagandharishta-T and Ashwagandharishta-M respectively. Super-oxide anion scavenging activity and lipid per-oxidation assay were carried out to evaluate the antioxidant potential of Ashwagandharishta-T and Ashwagandharishta-M. The antioxidant activity of Ashwagandharishta-T and Ashwagandharishta-M was found increased in concentration dependent manner in both the in vitro antioxidant activity models as super-oxide radical scavenging activity and lipid per-oxidation assay. Ashwagandharishta-T and Ashwagandharishta-M showed significant scavenging of super-oxide radical and showed IC50 91.32 and 99.39 μg/ml respectively. Ashwagandharishta-T and Ashwagandharishta-M also inhibited the ferrous sulphate induced lipid peroxidation in dose dependent manner and showed inhibitory concentration (IC50) 181.88 and 191.05 μg/ml respectively. Marketed Ashwagandharishta also showed a rich concentration of total phenolics and flavonoids and showed dose dependent antioxidant activity in both the models. Thus, the results obtained in this study indicate that Ashwagandharishta-T and Ashwagandharishta-M can be a promising source of natural antioxidant.Keywords
Total Phenolics, Flavonoids, Antioxidant Potential, Ashwagandharishta.- Evaluation of Cardioprotective Potential of Drakshasava in Induced Diabetic Condition
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Authors
Affiliations
1 Department of Pharmacognosy and Phytochemistry, IIMT College of Medical Sciences, Meerut, (U.P.), IN
2 Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat, IN
3 IIMT College of Medical Sciences, Meerut, (U.P.), IN
1 Department of Pharmacognosy and Phytochemistry, IIMT College of Medical Sciences, Meerut, (U.P.), IN
2 Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, Gujarat, IN
3 IIMT College of Medical Sciences, Meerut, (U.P.), IN
Source
Research Journal of Pharmacognosy and Phytochemistry, Vol 5, No 2 (2013), Pagination: 87-93Abstract
The objective of the present study was to evaluate the effect of Drakshasava- T and Drakshasava-M prepared by traditional and modern methods respectively and marketed Drakshasava on fasting blood glucose and serum lipid profile in alloxan induced diabetic rats. Oral administration of Drakshasava-T, Drakshasava-M and marketed Drakshasava (2 ml/kg p.o.) for 21 days caused a significant decrease in fasting blood glucose (FBG) and showed significant rise in blood glutathione level (GSH) in diabetic rats. Glibenclamide was used as a standard antidiabetic drug (10 mg/kg, p.o). These preparations also caused significant reduction in serum cholesterol, LDL and triglycerides and showed significant rise in serum HDL level in diabetic albino rats. Thus all these preparations were able to maintain the tested parameters near to the normal level significantly.Keywords
Cardiovascular Risk, Blood Glucose, Anti-Diabetic Potential, Glutathione, Lipid Profile, Drakshasava, Alloxan.- Total Phenolics and Flavonoids and Antioxidant Potential of Draksharishta Prepared by Traditional and Modern Methods
Abstract Views :186 |
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Authors
Affiliations
1 Department of Pharmacognosy, Shri Sarvajanik Pharmacy College, Mehsana-384001, Gujarat, IN
2 Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711,Gujarat, IN
1 Department of Pharmacognosy, Shri Sarvajanik Pharmacy College, Mehsana-384001, Gujarat, IN
2 Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711,Gujarat, IN
Source
Research Journal of Pharmacognosy and Phytochemistry, Vol 4, No 5 (2012), Pagination: 244-249Abstract
The objective of the present study was to estimate the total phenolic content as well as flavonoids in Draksharishta-T and Draksharishta-M prepared by traditional and modern methods respectively and in its marketed preparation and also to evaluate the antioxidant activity of these test preparations on two different in vitro antioxidant activity models. Total phenolic content was determined colorimetrically using Folin-Ciocalteu reagent and was found 0.0967 and 0.0961 %w/w gallic acid equivalent in Draksharishta-T and Draksharishta-M respectively. Total flavonoid content was determined by aluminium chloride method and was found 0.01163 and 0.01129 %w/w quercetin equivalent in Draksharishta-T and Draksharishta-M respectively. Super-oxide anion scavenging activity and lipid per-oxidation assay were carried out to evaluate the antioxidant potential of Draksharishta-T and Draksharishta-M. The antioxidant activity of Draksharishta-T and Draksharishta-M was found increased in concentration dependent manner in both the in vitro antioxidant activity models as super-oxide radical scavenging activity and lipid per-oxidation assay. Draksharishta-T and Draksharishta-M showed significant scavenging of super-oxide radical and showed IC50 138.06 and 145.35 μg/ml respectively. Draksharishta-T and Draksharishta-M also inhibited the ferrous sulphate induced lipid peroxidation in dose dependent manner and showed inhibitory concentration (IC50) 230.03 and 236.11 μg/ml respectively. Marketed Draksharishta also showed a rich concentration of total phenolics and flavonoids and showed dose dependent antioxidant activity in both the models. Thus, the results obtained in this study indicate that Draksharishta-T and Draksharishta-M can be a promising source of natural antioxidant.Keywords
Total Phenolics, Flavonoids, Antioxidant Potential, Draksharishta.- Quantification of Quercetin and Rutin in Arjunarishta Prepared by Traditional and Modern Methods by Validated HPTLC Densitometry
Abstract Views :145 |
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Authors
Affiliations
1 Department of Pharmacognosy, Shri Sarvajanik Pharmacy College, Mehsana-384001, IN
2 Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, IN
1 Department of Pharmacognosy, Shri Sarvajanik Pharmacy College, Mehsana-384001, IN
2 Department of Pharmacognosy, Shri S. K. Patel College of Pharmaceutical Education and Research, Kherva-382711, IN